CRISPETa

Paste your target regions in BED (example) or UCSC (example) format into the box. For definition of target region, see graphic below. You can also paste your design region as a FASTA sequence, using the next tab.

Genome:

BED file:

Options

Design settings

Positive mask:

Negative mask:

Upstream design region (bp): Downstream design region (bp):

Upstream exclude region (bp): Downstream exclude region (bp):

sgRNA scoring

Individual score:

Paired score:

Score combination: SumProduct

Output

Number of sgRNAs per target: Ranking method:

Construct method: Diversity: